Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

نویسندگان

  • Koen Nauwelaerts
  • Karen Vastmans
  • Matheus Froeyen
  • Veerle Kempeneers
  • Jef Rozenski
  • Helmut Rosemeyer
  • Arthur Van Aerschot
  • Roger Busson
  • Jeffrey C Lacey
  • Ekaterina Efimtseva
  • Sergey Mikhailov
  • Eveline Lescrinier
  • Piet Herdewijn
چکیده

A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction.

Incorporation of a disaccharide nucleoside into double-stranded DNA can be considered as a chemical (non-enzymatic) alternative for site-specific cleavage of DNA. Crystals of the sequence d(CGCGAATT*CGCG), where * is an incorporated ribose, were obtained by hanging-drop vapour diffusion and diffracted to 2.6 A. The crystals belong to the orthorhombic space group P222(1), with unit-cell paramete...

متن کامل

I-38: Chromosome Instability in The Cleavage Stage Embryo

Recently, we demonstrated chromosome instability (CIN) in human cleavage stage embryogenesis following in vitro fertilization (IVF). CIN not necessarily undermines normal human development (i.e. when remaining normal diploid blastomeres develop the embryo proper), however it can spark a spectrum of conditions, including loss of conception, genetic disease and genetic variation development. To s...

متن کامل

I-35: Genetic Aberrations in Early Development:The Origins and The Fates

Genetic aberrations are commonly seen in human preimplantation embryos. Non-disjunction and premature division of a chromosome are common in both meiosis and mitosis divisions. The expected result for meiotic aneuploidies is full aneuploidy in the later stages whereas mosaicism is the most frequent event in the cleavage and blastocyst stages. The main causes for mosaicism are post-zygotic event...

متن کامل

One-pot Synthesis of Amidoalkyl Naphthol Derivatives as Potential Nucleoside Antibiotics and HIV Protease Inhibitors using Nano-SnO2 as an Efficient Catalyst

An  efficient  three-component  one-pot  synthesis  of 1-amidoalkyl-2-naphthols  from  2-naphthol, aldehydes, and acetamide using nano-SnO2as catalyst is described. The reactions were carried out at 80oC under water-solvent media. The structures of the compounds were characterized by IR, 1HNMR, 13C-NMR,  and  Mass  spectra  and  by  elemental  analysis.  The  advantages  of  the  effective meth...

متن کامل

Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage

Objective: Berberis vulgaris contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of B. vulgaris on DNA cleavage. <span style="font-size: medium;"...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Nucleic acids research

دوره 31 23  شماره 

صفحات  -

تاریخ انتشار 2003